Opened 18 years ago
Closed 18 years ago
#467 closed defect (fixed)
GPR import problem
Reported by: | base | Owned by: | Johan Enell |
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Priority: | major | Milestone: | BASE 2.2 |
Component: | web | Version: | 2.1.1 |
Keywords: | gpr | Cc: |
Description
Hello, I couldn't use neither the Cy5/cy3 gpr import plugin or the Cy3/cy5 plugin. I checked and found out that there was a problem in the plugin configuration. The wavelengths of the dyes are inversed. cy5 = 635 whereas cy3=532
So the data header line for Cy3/CY5 should look like this:
"Block"\t"Column"\t"Row"\t"Name"\t"ID".*"Ratio of Medians \(532\/635\)".*
whereas the data header line for the Cy5/Cy3 should look like this:
"Block"\t"Column"\t"Row"\t"Name"\t"ID".*"Ratio of Medians \(635\/532\)".*
This fixed the problem. That's it.
Change History (2)
comment:1 by , 18 years ago
comment:2 by , 18 years ago
Milestone: | BASE 2.1.2 → BASE 2.2 |
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Resolution: | → fixed |
Status: | new → closed |
This has already been fixed and will be included in the BASE 2.2 release.
Ooops, I forgot to add another change to the Cy5/Cy3 plugin. Both the columns RATIO SD and Log Ratio should be as follows.
\Ratios SD (532/635)\ \Log Ratio (532/635)\
\Rgn R2 (635/532)\ "The 2 must not be and exp number" . This one applies to both plugins.
Please check both plugins for consistency.