error when hybridising multiple arrays on one slide (Agilent 4x44k)

[base]

I am using the agilent 4x44k arrays (4 arrays per slide), and cant seem to be able to enter hybridisations. This is what I do:

• I have array designs with 4 arrays per slide and I have a batch with 20 slides of that design

• I create a new hybridisation whereby I enter '4' Arrays and pick 'slideXY' on the 'hybridisation' tab, and array index:'1' on the 'labeled extracts' tab.

• So far so good, but if I want to hybridise a new sample on the same slide (I do the same as above but use array index: 2) I get the error message below:

Best Klemens

Version BASE 2.9.3 (build #4774; schema #65) Web server Apache Tomcat/6.0.18 Database Server MySQL 5.0.45-Max Database Dialect org.hibernate.dialect.MySQLInnoDBDialect JDBC Driver com.mysql.jdbc.Driver (version 5.1) Java runtime Java(TM) SE Runtime Environment (1.6.0_12-b04), Sun Microsystems Inc. Operating system Linux amd64 2.6.22.19-0.2-default Memory Total: 135.6 MB Free: 10.5 MB Max: 444.5 MB Browser Mozilla/5.0 (Windows; U; Windows NT 5.1; en-GB; rv:1.9.0.8) Gecko/2009032609 Firefox/3.0.8 Error message Duplicate entry '102' for key 2 Stack trace

DatabaseException ...at HibernateUtil.saveData(HibernateUtil.java:1134) ...at DbControl.commit(DbControl.java:394) ...at org.apache.jsp.views.hybridizations.index_jsp._jspService(index_jsp.java:238) ...at org.apache.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) ...at javax.servlet.http.HttpServlet.service(HttpServlet.java:717) ...at org.apache.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:374) ...at org.apache.jasper.servlet.JspServlet.serviceJspFile(JspServlet.java:342) ...at org.apache.jasper.servlet.JspServlet.service(JspServlet.java:267)

...at javax.servlet.http.HttpServlet.service(HttpServlet.java:717) ...at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:290) ...at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206) ...at net.sf.basedb.clients.web.servlet.CharacterEncodingFilter.doFilter(CharacterEncodingFilter.java:70) ...at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235) ...at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206) ...at org.apache.catalina.core.StandardWrapperValve.invoke(StandardWrapperValve.java:233) ...at org.apache.catalina.core.StandardContextValve.invoke(StandardContextValve.java:191) ...at org.apache.catalina.core.StandardHostValve.invoke(StandardHostValve.java:128) ...at org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:102) ...at org.apache.catalina.core.StandardEngineValve.invoke(StandardEngineValve.java:109) ...at org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:286) ...at org.apache.coyote.http11.Http11Processor.process(Http11Processor.java:845) ...at org.apache.coyote.http11.Http11Protocol$Http11ConnectionHandler.process(Http11Protocol.java:583) ...at org.apache.tomcat.util.net.JIoEndpoint$Worker.run(JIoEndpoint.java:447) ...at java.lang.Thread.run(Thread.java:619)

Caused by: com.mysql.jdbc.exceptions.jdbc4.MySQLIntegrityConstraintViolationException: Duplicate entry '102' for key 2 ...at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native Method) ...at sun.reflect.NativeConstructorAccessorImpl.newInstance(NativeConstructorAccessorImpl.java:39) ...at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27) ...at java.lang.reflect.Constructor.newInstance(Constructor.java:513) ...at com.mysql.jdbc.Util.handleNewInstance(Util.java:406) ...at com.mysql.jdbc.Util.getInstance(Util.java:381) ...at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:1015) ...at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:956) ...at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:3491) ...at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:3423) ...at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java:1936) ...at com.mysql.jdbc.ServerPreparedStatement.serverExecute(ServerPreparedStatement.java:1328) ...at com.mysql.jdbc.ServerPreparedStatement.executeInternal(ServerPreparedStatement.java:838) ...at com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:2019) ...at com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:1937) ...at com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:1922) ...at com.mchange.v2.c3p0.impl.NewProxyPreparedStatement.executeUpdate(NewProxyPreparedStatement.java:105) ...at org.hibernate.id.IdentityGenerator$GetGeneratedKeysDelegate.executeAndExtract(IdentityGenerator.java:94) ...at org.hibernate.id.insert.AbstractReturningDelegate.performInsert(AbstractReturningDelegate.java:57) ...at org.hibernate.persister.entity.AbstractEntityPersister.insert(AbstractEntityPersister.java:2186) ...at org.hibernate.persister.entity.AbstractEntityPersister.insert(AbstractEntityPersister.java:2666) ...at org.hibernate.action.EntityIdentityInsertAction.execute(EntityIdentityInsertAction.java:71) ...at org.hibernate.engine.ActionQueue.execute(ActionQueue.java:279) ...at org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:321) ...at org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:204) ...at org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:130) ...at org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:210) ...at org.hibernate.event.def.DefaultSaveEventListener.saveWithGeneratedOrRequestedId(DefaultSaveEventListener.java:56) ...at org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:195) ...at org.hibernate.event.def.DefaultSaveEventListener.performSaveOrUpdate(DefaultSaveEventListener.java:50) ...at org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:93) ...at org.hibernate.impl.SessionImpl.fireSave(SessionImpl.java:562) ...at org.hibernate.impl.SessionImpl.save(SessionImpl.java:550) ...at org.hibernate.impl.SessionImpl.save(SessionImpl.java:546) ...at HibernateUtil.saveData(HibernateUtil.java:1129) ...at DbControl.commit(DbControl.java:394) ...at org.apache.jsp.views.hybridizations.index_jsp._jspService(index_jsp.java:238) ...at org.apache.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) ...at javax.servlet.http.HttpServlet.service(HttpServlet.java:717) ...at org.apache.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:374) ...at org.apache.jasper.servlet.JspServlet.serviceJspFile(JspServlet.java:342) ...at org.apache.jasper.servlet.JspServlet.service(JspServlet.java:267) ...at javax.servlet.http.HttpServlet.service(HttpServlet.java:717) ...at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:290) ...at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206) ...at net.sf.basedb.clients.web.servlet.CharacterEncodingFilter.doFilter(CharacterEncodingFilter.java:70) ...at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235) ...at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206) ...at org.apache.catalina.core.StandardWrapperValve.invoke(StandardWrapperValve.java:233) ...at org.apache.catalina.core.StandardContextValve.invoke(StandardContextValve.java:191) ...at org.apache.catalina.core.StandardHostValve.invoke(StandardHostValve.java:128) ...at org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:102) ...at org.apache.catalina.core.StandardEngineValve.invoke(StandardEngineValve.java:109) ...at org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:286) ...at org.apache.coyote.http11.Http11Processor.process(Http11Processor.java:845) ...at org.apache.coyote.http11.Http11Protocol$Http11ConnectionHandler.process(Http11Protocol.java:583) ...at org.apache.tomcat.util.net.JIoEndpoint$Worker.run(JIoEndpoint.java:447) ...at java.lang.Thread.run(Thread.java:619)

[Nicklas Nordborg]

A slide can only be associated with a single hybridization. The proper way for this case is to create one hybridization that is linked to the slide and to all (four) labeled extracts, each one with a different index (1-4).