id,summary,reporter,owner,description,type,status,priority,milestone,component,version,resolution,keywords,cc 837,Lowess plugin. Error: Sum of weigths in line_fit is not positive,base,everyone,"Sorry if this should go to baseplugins trac, but as a core plugin maybe it should go here. I just ran BASE2 lowess on 54 bioassays and got the error below (right at the end of the job). I think the problem could relate to having three different array designs in the same experiment. When I run on a subset with the same array design it works fine (I just ran it again to check). cheers, Bob. {{{ View job -- Run plugin: Normalization: Lowess Name Run plugin: Normalization: Lowess Description Priority 5 (1 = highest, 10 = lowest) Status Error: Sum of weigths in line_fit is not positive Percent complete 100% Created 2007-11-22 11:58:24 Started 2007-11-22 11:58:39 Ended 2007-11-22 12:10:50 Server bio-iisrv1.bio.ic.ac.uk User Bob MacCallum Experiment - none - Plugin Normalization: Lowess Configuration - none - net.sf.basedb.core.BaseException: Sum of weigths in line_fit is not positive at net.sf.basedb.plugins.LowessNormalization.weightedLeastSquaresRegression(LowessNormalization.java:574) at net.sf.basedb.plugins.LowessNormalization.lowess(LowessNormalization.java:487) at net.sf.basedb.plugins.LowessNormalization.run(LowessNormalization.java:297) at net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java:89) at net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java:421) at java.lang.Thread.run(Thread.java:619) Job parameters Blockgroup size 1 Child description Child name All hybs - bg subtracted - no bad spots - lowess Minimum log(intensity) step 0.1 Window size (fraction of points) 0.33 Iterations 4 Source bioassay set All hybs - bg subtracted - no bad spots }}} BASE version info: {{{ Version BASE 2.4.6pre (build #3938; schema #40) Web server Apache Tomcat/5.5.20 Database Server MySQL 5.0.21-max-log Database Dialect org.hibernate.dialect.MySQLInnoDBDialect JDBC Driver com.mysql.jdbc.Driver (version 5.0) Java runtime Java(TM) SE Runtime Environment (1.6.0-b105), Sun Microsystems Inc. Operating system Linux amd64 2.6.16.53-0.16-smp Memory Total: 359.1 MB Free: 99.1 MB Max: 910.3 MB }}} ",defect,closed,trivial,BASE 2.6,coreplugins,,fixed,,