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2 | The BioRobotics 'TAM' format explained (an asterisk denotes an explanation rather than a component of the file format): |
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3 | |
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4 | [FileInformation] |
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5 | FileFormat=,1.0 *Version number of the file format |
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6 | FormatName=,TAM *File format extension |
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7 | GeneratedBy=,TAS2.1.5.16 *Arrayer software and version |
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8 | BlockCount=,4 *Number of meta-grids |
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9 | SpotSize=,180 *Estimated mean spot diameter |
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10 | |
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11 | |
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12 | [Block1] *Sub-grid being examined |
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13 | MetaGridX=,1 *Meta-grid X-axis co-ordinate |
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14 | MetaGridY=,1 *Meta-grid Y-axis co-ordinate |
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15 | OriginX=,9000 *Distance from top left edge of slide to centre of top left spot |
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16 | OriginY=,35300 *Distance from top left edge of slide to centre of top left spot |
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17 | BlockSizeX=,4 *Number of spots in each row |
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18 | BlockSizeY=,4 *Number of spots in each column |
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19 | SpacingX=,400 *X-axis centre-to-centre spot spacing |
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20 | SpacingY=,400 *X-axis centre-to-centre spot spacing |
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21 | *Information is repeated for each sub-grid on the slide |
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22 | |
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23 | [mapping] *'comma separated value' spreadsheet that maps clones to wells |
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24 | 1,1,1,1,,1,9,4,"FC1269","1036",1, {},FC1269,1036,TST101,I,4 |
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25 | 1,1,1,2,,1,1,4,"FC1237","1004",1, {},FC1237,1004,TST101,A,4 |
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26 | 1,1,1,3,,1,9,2,"FC1267","1034",1, {},FC1267,1034,TST101,I,2 |
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27 | 1,1,1,4,,1,1,2,"FC1235","1002",1, {},FC1235,1002,TST101,A,2 |
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28 | 1,1,2,1,,1,11,4,"FC1277","1044",1, {},FC1277,1044,TST101,K,4 |
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29 | |
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30 | *Spreadsheet abbreviated for brevity. |
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31 | *Each row of data relates to each spot on the microarray. |
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32 | *Origin is top left of slide for all measurements. |
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33 | |
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34 | *Column 01: Meta-grid X-axis co-ordinate |
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35 | *Column 02: Meta-grid Y-axis co-ordinate |
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36 | *Column 03: Sub-grid Y-axis co-ordinate |
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37 | *Column 04: Sub-grid X-axis co-ordinate |
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38 | *Column 05: Plate Barcode |
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39 | *Column 06: Plate Number |
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40 | *Column 07: Row Number |
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41 | *Column 08: Column Number |
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42 | *Column 09: Sample Name |
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43 | *Column 10: Sample ID |
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44 | *Column 11: Block number |
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45 | *Column 12: Termination of additional fields |
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46 | *Column 13 to End: Source data from the operator (See Example Array Input File) |
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47 | --- |
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