1 | <?xml version="1.0" encoding="UTF-8"?>
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2 | <!DOCTYPE configfile SYSTEM "plugin-configuration-file.dtd"><configfile>
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3 | <configuration pluginClassName="se.uu.lcb.plugins.PluginExecuter">
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4 | <configname>Normalization: Within arrays</configname>
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5 | <description>This plug-in normalizes log-ratios for each array individually by various methods for averaging log-ratios to zero within each array or sub-array. The print-tip version of this normalization, which is a local fitting of the regression line, is usually preferred since it corrects for both spatial effects and dye biases. All available methods are listed below:
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6 | - Median: The median log-ratio is centered on zero.
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7 | - Loess: All intensities on the array are fitted to a regression line, so that dye biases are removed. Yang et al (2001, 2002)
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8 | - Print-tip loess: Loess normalization is perfromed within each sub array, so that both spatial effects and dye biases are removed. Yang et al (2001, 2002)
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9 | - Composite: This method is not yet operational.
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10 | - Robust spline: Robustly fitted regression splines and empirical Bayes shrinkage.</description>
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11 | <parameter>
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12 | <name>leaveStdin</name>
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13 | <label>Leave stdin.txt</label>
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14 | <description>If the file stdin.txt should be removed after the plug-in execution or not</description>
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15 | <class>java.lang.Boolean</class>
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16 | <value>false</value>
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17 | </parameter>
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18 | <parameter>
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19 | <name>leaveStdout</name>
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20 | <label>Leave stdout.txt</label>
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21 | <description>If the file stdout.txt should be removed after the plug-in execution or not</description>
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22 | <class>java.lang.Boolean</class>
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23 | <value>false</value>
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24 | </parameter>
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25 | <parameter>
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26 | <name>parameters</name>
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27 | <label>Job parameters</label>
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28 | <description>Job parameters</description>
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29 | <class>java.lang.String</class>
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30 | <value>fname\thidden\tnormalization.R\tfname</value>
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31 | <value>norm.method\tenum\tprinttiploess;printtiploess|loess;loess|median;median|composite;composite|robustspline;robustspline\tNormalization method</value>
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32 | <value>smooth.param\tfloat\t0.3\tProp. size of local regression moving window</value>
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33 | <value>specifyLayout\tbool\ttrue\tSpecify array layout dimensions?</value>
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34 | <value>ngrid.r.in\tint\t0\tGrid rows per slide</value>
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35 | <value>ngrid.c.in\tint\t0\tGrid columns per slide</value>
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36 | <value>nspot.r.in\tint\t0\tSpot rows per print-tip group</value>
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37 | <value>nspot.c.in\tint\t0\tSpot columns per print-tip group</value>
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38 | <value>use.weights\tbool\tfalse\tAssociate quality weights with flag values?</value>
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39 | <value>weight.0\tfloat\t\tFlag values with weight 0</value>
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40 | <value>weight.25\tfloat\t\tFlag values with weight 0.25</value>
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41 | <value>weight.5\tfloat\tNA\tFlag values with weight 0.5</value>
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42 | <value>weight.75\tfloat\tNA\tFlag values with weight 0.75</value>
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43 | <value>debug.level\thidden\t0\tdebug.level</value>
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44 | </parameter>
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45 | <parameter>
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46 | <name>usedFields</name>
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47 | <label>Spot fields</label>
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48 | <description>Spot fields</description>
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49 | <class>java.lang.String</class>
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50 | <value>intensity1</value>
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51 | <value>intensity2</value>
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52 | <value>raw.flags</value>
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53 | </parameter>
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54 | <parameter>
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55 | <name>displayAssayOrder</name>
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56 | <label>Display assay order</label>
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57 | <description>Display assay order</description>
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58 | <class>java.lang.Boolean</class>
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59 | <value>false</value>
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60 | </parameter>
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61 | <parameter>
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62 | <name>executablePath</name>
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63 | <label>Path to executable</label>
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64 | <description>Path to executable file</description>
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65 | <class>java.lang.String</class>
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66 | <value>/Sites/plugins/base1/bin</value>
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67 | </parameter>
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68 | <parameter>
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69 | <name>isOneChannel</name>
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70 | <label>One channel data</label>
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71 | <description>One channel data</description>
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72 | <class>java.lang.Boolean</class>
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73 | <value>false</value>
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74 | </parameter>
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75 | <parameter>
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76 | <name>usedColumns</name>
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77 | <label>Reporter columns</label>
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78 | <description>Reporter columns</description>
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79 | <class>java.lang.String</class>
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80 | <value>rep.position</value>
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81 | <value>rep.id</value>
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82 | </parameter>
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83 | <parameter>
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84 | <name>execName</name>
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85 | <label>Name of executable</label>
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86 | <description>Name of executable</description>
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87 | <class>java.lang.String</class>
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88 | <value>R2.pl</value>
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89 | </parameter>
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90 | </configuration>
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91 | </configfile>
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